Laboratory and Analytical Methods


 * 1) Simple (glass) laboratory equipment and how to use it
 * 2) Laboratory safety
 * 3) Size exclusion chromatography
 * UV-VIS Spectrometry
 * IR spectrometry
 * Centrifugation and Ultracentrifugation
 * 1) SDS PAGE technique: Proteins are treated with a material that gives them a charge in proportion to their mass. They are then placed at the starting line of a gel that has a current running through it. The molecules travel down the gel like electrons travel through an power line, this is called electrophoresis. Since the proteins have the same charge/mass ratio, they travel down the gel according to their size. The smallest proteins go the furthest. These results can then be compared in many different ways.
 * 2) *For more information see Details on Electrophoresis or Details on SDS-PAGE.
 * Chromatography
 * 1) Gas chromatography and mass spectrometry
 * Gamma counting
 * Beta counting
 * Enzyme assays
 * PCR
 * Metabolic pathways

Return to Biochemistry page