PlanetPhysics/George Palade

Brief summary
Dr. George E. Palade won the Nobel Prize in 1974. Born: November 19, 1912, at Jassy (Iashi), Romania Died: October 7, 2008 (aged 95) Citizenship: United States Nationality Romanian fields: cell biologist, biophysicist Alma mater: Carol Davila School of Medicine Known for: Rough ER Notable awards: 1974 Nobel Prize in Physiology or Medicine and the US National Medal of Science in 1986.

Science Awards
George Emil Palade (November 19, 1912 --- October 7, 2008) was a Romanian--American cell biologist and biophysicist. In 1974, he shared the Nobel Prize in Physiology or Medicine with Albert Claude and Christian de Duve, for discovering the cellular vacuole.

Palade also received the U.S. National Medal of Science in Biological Sciences for "pioneering discoveries of a host of fundamental, highly organized structures in living cells..." in 1986,(National Medal of Science), and was previously elected a Member of the US National Academy of Science in 1961.

Contents

 * 1 Brief Summary
 * 1.1. Science Awards
 * 1.2. Contents


 * 2. Biography


 * 2.1 Research note: Palade's coworkers and approach in the 1960s


 * 3. References


 * 4. Sources


 * 5 Relevant Research Areas


 * 6 External links


 * 7. Note

Biography
George Emil Palade was born on November 19, 1912 at Jassy (Iasi), in Romania; his father was a Professor of Philosophy at the University and his mother was a high school teacher. Both parents strongly encouraged George to further develop his abilities through higher education at university. George E. Palade received his M.D. in 1940 from the Carol Davila School of Medicine of the University of Bucharest, Romania. He was a member of the faculty of that famous school until 1945 when he went to the United States for postdoctoral studies. There, he joined Prof. Albert Claude at the Rockefeller Institute for Medical Research [1].

In 1952, Palade became a naturalized citizen of the United States. He was a Professor at the Rockefeller Institute (1958-1973), Yale University Medical School (1973-1990), and University of California, San Diego (1990-2008). At UCSD, Palade was Professor of Medicine in Residence (Emeritus) in the Department of Cellular and Molecular Medicine, as well as a Dean for Scientific Affairs (Emeritus), in the School of Medicine at La Jolla, California.[2] In 1970, he was awarded[3] the Louisa Gross Horwitz Prize from Columbia University together with Renato Dulbecco co-winner of 1974 Nobel Prize in Physiology or Medicine "for discoveries concerning the functional organization of the cell that were seminal events in the development of modern cell biology.",[4] related to his previous research carried out at the Rockefeller Institute for Medical Research[5]. His Nobel lecture, delivered on December 12, 1974, was entitled: "Intracellular Aspects of the Process of Protein Secretion" [6], published in 1992 by the Nobel Prize Foundation [7], [8]. In 1988 he was elected an Honorary Member of the American-Romanian Academy of Arts and Sciences (ARA).

Palade was the first Chairman of the Department of Cell Biology at Yale University. Presently, the Chair of Cell Biology at Yale is named the "George Palade Professorship".

At the Rockefeller Institute for Medical Research, Palade used electron microscopy to study the internal organization of such cell structures as ribosomes, mitochondria, chloroplasts, the Golgi apparatus, and others. His most important discovery was made while using an experimental strategy known as a pulse-chase analysis. In the experiment Palade and his colleagues were able to confirm an existing hypothesis that a secretory pathway exists and that the Rough ER and the Golgi apparatus function together.

He focused on Weibel-Palade bodies (a storage organelle unique to the endothelium, containing von Willebrand factor and various proteins) which he described together with the Swiss anatomist Ewald R. Weibel. [9]

Palade is survived by his wife Marilyn Farquhar, a cell biologist at the University of California, San Diego[10].

Research note: Palade's coworkers and approach in the 1960s
The following is a concise excerpt from Palade's Autobiography appearing in the Nobel Award documents[1]

"In the 1960s, I continued the work on the secretory process using in parallel or in succession two different approaches. The first relied exclusively on cell fractionation, and was developed in collaboration with Philip Siekevitz, Lewis Greene, Colvin Redman, David Sabatini and Yutaka Tashiro; it led to the characterization of the zymogen granules and to the discovery of the segregation of secretory products in the cisternal space of the endoplasmic reticulum. The second approach relied primarily on "radioautography'', and involved experiments on intact animals or pancreatic slices which were carried out in collaboration with Lucien Caro and especially James Jamieson. This series of investigations produced a good part of our current ideas on the synthesis and intracellular processing of proteins for export. A critical review of this line of research is presented in the Nobel Lecture.'' [11].

One notes also that the Nobel Prize in Chemistry was awarded in 2009 to Drs. Venkatraman Ramakrishnan, Thomas A. Steitz and Ada E. Yonath "for studies of the structure and function of the "ribosome , discovered by Dr. George Emil Palade[12].

Relevant Research Areas

 * Cell biology


 * Ribosomes


 * RNAs


 * Protein synthesis


 * Biosynthesis


 * Molecular biology


 * Biophysics


 * Nobel laureates


 * Electron microscopy


 * Photosynthesis

Note
This entry is based in part, and has significant additions to, as well as changes from, a related web entry.

Literature Cited
1. Claude, A., J. Exper. Med., 84 (1946) 51, 61.

2. Hogeboom, G. H., Schneider, W. C., and Palade, G. E., J. Biol. Chem., 172 (1948) 619.

3. Kennedy, E. P., and Lehninger, A. L., J. Biol. Chem., 179 (1949) 957.

4. Palade, G. E., J. Biophys. Biochem. Cytol., I (1955) 59.

5. Palade, G. E., in Microsomal particles and protein synthesis, Roberts, R. B., editor, Pergamon Press, 1958.

6. Roberts, R. B. in Introduction to Microsomal particles and protein synthesis, Roberts, R. B., editor, Pergamon Press, 1958.

7. Porter, K. R., Claude, A. and Fullam, E., .J. Exper. Med. 81 (1945) 233.

8. Porter, K. R., J. Exper. Med., 97 (1953) 727.

9. Palade, G. E., and Porter, K. R., J. Exper. Med., ZOO (1954) 641.

10. Porter, K. R., and Palade, G. E., Biophys. Biochem. Cytol., 3 (1957) 269.

11. Palade, G. E., J. Biophys. Biochem. Cytol., 2 (suppl.) (1956) 85.

12. Siekevitz, P., J. Biol. Chem., 195 (1952) 549.

13. Northrop, J. H., Kunitz, M., and Herriott, R. M., Crystalline Enzymes, Columbia University Press ( 1948).

14. Palade, G. E., and Siekevitz, P., J. Biophys. Biochem. Cytol., 2 ( 1956) 171, 671.

15. Siekevitz, P., and Palade, G. E., J. Biophys. Biochem. Cytol., 4 (1958) 203, 309, 557; 5 (1959) 1.

16. Care, L. G., and Palade, G. E., J. Cell Biol., 20 (1964) 473.

17. Redman, C. M., Siekevitz, P., and Palade, G. E., J. Biol. Chem., 242 (1966) 1150.

18. Jamieson, J. D., and Palade, G. E., J. Cell Biol., 34 (1967) 577.

19. Castle, J. D., Jamieson, J. D., and Palade, G. E., J. Cell Biol., 53 (1972) 290.

20. Scheele, G. A., and Palade, G. E., J. Biol Chem., 250 (1975) 2660.

21. Siekevitz, P., and Palade, G. E., J. Biophys. Biochem. Cytol., 7 (1960) 619, 631.

22. Redman, C. M., J. Biol. Chem., 244 (1969) 4308.

23. Hicks, S. J., Drisdale, J. W., and Munro, H. N., Science (Washington) 164 ( 1969) 584.

24. Milstein, C., Brownlee, G .G., Harrison, T. M., and Mathews, M. B., Nature, 239 (1972) 117.

25. Blobel, G., and Sabatini, D. D., in Biomembranes 2 (1971) 193; L. A. Menton 204 Physiology or Medicine 1974, Editor, Plenum Publish. Co., New York.

26. Dallner, G., Siekevitz, P., and Palade, G. E., J. Cell Biol., 30 (1966) 73, 97.

27. Chua, N. H., Blobel, G., Siekevitz, P., and Palade, G. E., Proc. Nat. Acad. Sci., U.S.A., 70 (1973) 1554.

28. Kessel, R. G., Zeitschr. Zellforschung, 89 (1968) 17.

29. Redman, C. M., and Sabatini, D. D., Proc. Nat. Acad. Sci., U.S.A. 56 (1966) 608.

30. Blobel, G., and Sabatini, D. D., J. Cel1 Biol., 45 (1970) 146.

31. Sabatini, D. D., Tashiro, Y., and Palade, G. E., J. Mol. Biol., 19 (1966) 503.

32. Tedeschi, H., James, J. M., and Anthony, W., J. Cell Biol., 18 (1963) 503. 33. Anfinson, C. B., Harvey Lectures 62 (1966) 95.

34. Olsen, B. R., Berg, R. A., Kishida, Y., and Prockop, D. J., Science (Washington)182 (1973) 825.

35. Molnar, J., Robinson, G. B., and Winzler, R. J., J. Biol. Chem., 240 (1965) 1882.

36. Mach, B., Koblet, H., and Gras, D., Proc. Nat. Acad. Sci., U.S.A. 59 (1968) 445.

37. Grant, M. G., and Prockop, D. J., New England J. Med., 286 (1972) 194.

38. Bainton, D. F., and Farquhar, M. G., J. Cell Biol., 39 (1968) 299 and 45 (1970)54.

39. Herzog, V., and Miller, F., Z. Zellforsch. Mikrosk. Anat., 107 (1970) 403.

40. Lisowska-Berstein, B., Lamm, M. E., and Vassali, P., Proc. Nat. Acad. Sci., U.S.A. 66 (1970) 425.

41. Rogers, M. J., and Strittmatter, P., J. Biol. Chem. 249 (1974) 895, 5565.

42. Jamieson, J. D., and Palade, G. E., J. Cell Biol., 34 (1967) 597.

43. Jamieson, J. D., and Palade, G. E., J. Cell Biol., 39 (1968) 589.

44. Swenson, R. M., and Kern, M., Proc. Nat. Acad. Sci., U.S.A. 57 (1967) 417.

45. Wuhr, P., Herscovics, A., and Leblond, C. P., J. Cell Biol., 43 (1969) 289.

46. Haddad, A., Smith, M. D., Herscovics, A., Nadler, N. J., and Leblond, C. P., J. Cell Biol., 49 ( 1971) 856.

47. Zagury, D. Uhr, J. W., Jamieson, J. D., and Palade, G. E., J. Cell Biol., 46 (1970) 52.

48. Hopkins, C. R., and Farquhar, M. G., J. Cell Biol., 59 (1973) 276.

49. MorrC, D. J., Keenan, T. W., and Mollenhauer, II. H., in Advances in Cytopharmacology, Clementi, F., and Ceccarelli, B., editors, Raven Press, New York 1971.

50. Claude, A., J. Cell Biol., 47 (1970) 745.

51. Jamieson, J. D., and Palade, G. E., J. Cell Biol., 48 ( 1971) 503.

52. Hokin, L. E., Biochim. et Biophys. Acta 18 (1955) 379.

53. Greene, L. J., Hirs, C. H. W., and Palade, G. E., J. Biol. Chem. 238 (1963) 2054.

54. Berg, N. B., and Young, R. W., J. Cell Biol., 50 (1971) 469.

55. Young, R. W., J. Cell Biol., 57 (1973) 175.

56. Tartakoff, A. M., Greene, L. J., and Palade, G. E., J. Biol. Chem., 249 (1974) 7420.

57. Farquhar, M. G., Memoirs Soc. for Endocrinology, 19 (1971) 79.

58. Jamieson, J. D., and Palade, G. E., J. Cell Biol., 50 ( 1971) 135.

59. Neutra, M., and Leblond, C. P., J. Cell Biol., 30 (1966) 137.

60. Schachter, H., Jabbal, I., Hudgin, R. L., Pinteric, L., McGuire, J., and Roseman, S., J. Biol. Chem., 245 (1970) 1090.

61. Steiner, D. F., Clark, J. L., Nolan, C., Rubenstein, A. H., Margoliash, E., Melani, F., and Oyer, P. E., Proc. 13th Nobel Symposium, (1970) 123.

62. Dauwalder, M., Whaley, W. G., and Kephart, J. E., Subcell. Biochem., I (1972) 225.

63. Farquhar, M. G., Bergeron, J. J. M., and Palade, G. E., J. Cell Biol., 60 (1974) 8.

64. Ovtracht, L., and Thi\'ery, J. P., J. Microscopic, 15 (1972) 135. Intracellular Aspects of the Process of Protein Secretion 205

65. Novikoff, A. B., Essner, E., and Goldfischer, S., in The Interpretation of Ultrastructure, Harris, R. J. C., editor, Acad. Press, New York. (1962).

66. Friend, D. S., J. Cell Biol., 41 (1969) 269.

67. Bergeron, J. J. M., Ehrenreich, J. H., Siekevitz P., and Palade, G. E., J. Cell Biol., 59 (1973) 73.

68. Meldolesi, J., Jamieson, J. D., and Palade, G. E., J. Cell Biol., 49 ( 1971) 109--130.

69. Keenan, T. W., and Morr\'e, D. J., Biochemistry, 9 (1970) 19.

70. Fleischer, B., Fleischer, S., and Ozawa, H., J. Cell Biol., 43 (1969) 59.

71. Fleischer, B., and Fleischer, S., Biochim. Biophys. Acta, 229 (1970) 301.

72. Morr\'e, D. J., Hamilton, R. L., Mollenhauer, H. H., Mahley, R. W., Cunningham, W. P., Cheetham, R. D., and LeQuire, V. S., J. Cell Biol., 44 (1970) 484.

73. Ehrenreich, J. H., Bergeron, J. J. M., Siekevitz, P., and Palade, G. E., J. Cell Biol., 59 (1973) 45.

74. Fleischer, B., and Zambrano, F., J. Biol. Chem., 249 (1974) 5995.

75. Keller, P. J., and Cohen, E., J. Biol. Chem., 236 (1961) 1407.

76. Palla, J. C., These de Doctorat-\&s-Sciences, Marseilles, 1970.

77. Castle, J. D., Jamieson, J. D., and Palade, G. E., J. Cell Biol., 64 (1975) 182.

78. Kraehenbuhl, J. P., and Jamieson, J. D., Proc. Natl. Acad. Sci., U.S.A. 69 (1972) 1771.

79. Hay, E. D., and Dodson, Y. W., J. Cell Biol., 57 ( 1973) 190.

80. Painter, R. G., Tokuyashu, K. T., and Singer, S. J., Proc. Nat. Acad. Sci., U.S.A., 70 (1973) 1649.

81. Tartakoff, A. M., Jamieson, J. D., Scheele, G. A., and Palade, G. E., J. Biol. Chem., 250 (1974) 2671.

82. Palade, G. E., in Subcellular Particles, Hayashi, T., editor, Ronald Press, New York, 1959.

83. de Duve, C., in Lysosomes, Ciba Foundation Symposium, (1963) 126.

84. Ishikawa, A., J. Cell Biol., 24 (1965) 369.

85. Cuatrecasas, P., Proc. Nat. Acad. Sci., U.S.A. 68 (1971) 1264.

86. Douglas, W. W., and Rubin, R. P., J. Physiol., 159 ( 1961) 40 and 167 ( 1963) 288.

87. Douglas, W. W., Br. J. Pharmacol., 34 ( 1968) 451.

88. Schramm, M., Annu. Rev. Biochem., 36 (1967) 307.

89. Rasmussen, H., Science 170 (1970) 404.

90. Mathews, E. K., and Petersen, 0. H., J. Physiol., 231 (1973) 283.

91. Kulka, R. G., and Sternlicht, E., Proc. Nat. Acad. Sci., U.S.A. 61 ( 1968) 1123

92. Goodman, D. P. B., Rasmussen, H., DiBella, F., and Guthrow, C. E., Jr., Proc. Nat. Acad. Sci., U.S.A., 67 (1970) 652.

93. Dilorenzo, R. J., Walton, K. G., Curran, P. F., and Greengard, P., Proc. Nat. Acad. Sic., U.S.A., 70 (1973) 880.

94. Johnson, E. M., Ueda, T., Moeno, H., and Greengard, P., J. Biol. Chem., 247 (1972) 5650.

95. Lacy, P. E., Howell, S. L., Young, D. A., and Fink, C. J., Nature, 219 (1968) 1177.

96. Williams, J. A., and Wolff, J., J. Cell Biol., 54 (1972) 158.

97. LeMarchand, Y., Single, A., Assimacopoulos--Jeannet, F., Orci, L., Rouillier, C., and Jeanrenard, B., J. Biol. Chem., 248 (1973) 6862.

98. Stein, O., and Stein, Y., Biochim. Biophys. Acta 306 (1973) 142.

99. Redman, C. M., Banerjee, D., Howell, K., and Palade, G. E., J. Cell Biol., 64 (1975).

100. Stadler, J., and Franke, W. W., J. Cell Biol., 60 (1974) 297.

101. Wilson, L., Bamberg, J. R., Mizel, S. B., Grisham, L. M., and Creswell, K. M., 206 Physiology or Medicine 1974. Fed. Proc., 33 (1973) 158.

102. Heuser, J. E., and Reese, T. S., J. Cell Biol., 57 (1973) 315.

103. Ceccarelli, B., Hurlbut, W. P., and Mauro, A., J. Cell Biol., 57 (1973) 449.

104. Pelletier, G., J. Ultrastructure. Res. 43 (1973) 445.

105. Farquhar, M. G., Skutelsky, E., and Hopkins, C. R., in The Anterior Pituitary, Tixier-Vidal, A., and Farquhar, M. G., eds. Academic Press, New York 1975, p. 83.

106. Meldolesi, J., J. Cell Biol., 61 (1974) 1.

107. Castle, J. D., Thesis, Rockefeller University, 1974.

108. van Golde, L. M. G., Fleischer, B., and Fleischer, S., Biochim. Biophys. Acta 249 (1971) 318.

109. Ross, R., and Benditt, E., J. Cell Biol., 27 (1965) 83.

110. Weinstock, M., and Leblond, C. P., J. Cell Biol., 60(1974) 92.

111. Olsen, B. R., and Prockop, D. J., Proc. Nat. Acad. Sci., U.S.A. 71 (1974) 2033.

112. Zucker-Franklin, D., and Hirsch, J. G., J. Exper. Med., 120 ( 1964) 569.

113. Bainton, D. F., in Phagocytic mechanisms in health and disease, Williams, R. C.,and Fudenberg, H. H., eds. Intercontinental Medical Book Corp., New York, (1972).

114. Henson, P. M., J. Immunol. 107 (1971) 1547.

115. Cohn, Z. A., Fedorko, M. E., and Hirsch, J. G., J. Exp. Med., 123 ( 1966) 157.

116. Smith, R. E., and Farquhar, M. G., J. Cell Biol. 31 (1966) 319.

117. Novikoff, P. M., Novikoff, A. B., Quintana, N., and Hauw, J., J. Cell Biol., 50 (1971) 859.

118. Albersheim, P., Bauer, W. D., Keestra, K., and Talmadge, K. W., in Biogenesis of plant cell wall polysaccharides, Loewus, F., ed. Academic Press, New York (1973).

119. Fawcett, D. W., Biology of Reproduction, 2 (1970) 90.

120. Anderson, E., J. Cell Biol., 37 ( 1968) 514.

121. Szollosi, D., Anat. Record, 159 (1967) 431.

122. Douglas, W. W., and Poisner, A. M., J. Physiol. 172 ( 1964) 1.

123. Whittacker, V. P., in Advances in Cytopharmacology, 2 (1973) 311, Raven press, New York.

124. Ross, R., J. Cell Biol., 50 (1971) 172.

125. Hay, E. D., in The Epidermis, Montagna, W., and Lobitz, W. C., eds., Academic Press, New York, 1964, p. 97.