UTPA STEM/CBI Courses/Microbiology/Genetic Engineering Using Bacteria

Course Title: General Microbiology

Lecture Topic: Genetic Engineering in Bacteria

Instructor: Dr. Luis Materon

Institution: UTPA, Biology Department

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Challenge: There is currently a severe oil spill in the Gulf of Mexico that will affect wild life and business in the area. Task: Identify a gene that will degrade oil faster. The gene is to be inserted into a plasmid of an aquatic bacterial species such as Pelagibacter (The most abundant bacterial species in the ocean). The resulting recombinant bacteria having a new gene should accelerate the breakdown of oil on water and affected beaches. Adopt a protocol to isolate and insert a gene from Pseudomonas into Pelagibacter. Assess your contribution to reduce environmental damage from oil spill.


 * Backwards Design

Course Objectives


 * Primary Objectives- By the next class period students will be able to:
 * Understand
 * Discuss
 * Evaluate
 * Publish


 * Sub Objectives- The objectives will require that students be able to:
 * Consult protocols
 * Experiment with enzymes
 * Observe growth
 * Discuss results
 * Write report


 * Difficulties- Students may have difficulty:
 * Background knowledge in the molecular structure of DNA and other basic concepts
 * Getting access to lab after hours due to security regulations
 * Managing time
 * Reading results


 * Real-World Contexts- There are many ways that students can use this material in the real-world, such as:
 * Research-oriented job.
 * Teaching

Model of Knowledge


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 * Content Priorities
 * Enduring Understanding
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 * Important to Do and Know
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 * Worth Being Familiar with
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Assessment of Learning


 * Formative Assessment
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 * Homework (individual)
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 * Summative Assessment
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Legacy Cycle
OBJECTIVE

By the next class period, students will be able to: The objectives will require that students be able to:
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THE CHALLENGE

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GENERATE IDEAS

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MULTIPLE PERSPECTIVES

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RESEARCH & REVISE

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TEST YOUR METTLE

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GO PUBLIC

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Pre-Lesson Quiz

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Test Your Mettle Quiz

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